1PQ1
Crystal structure of Bcl-xl/Bim
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.100, 47.340, 49.280 |
| Unit cell angles | 90.00, 110.10, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.650 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | mouse Bcl-xl pdb entry 1PQ0 |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.110 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.062 | 0.218 |
| Number of reflections | 19877 | 1765 * |
| <I/σ(I)> | 8.6 | 2.7 |
| Completeness [%] | 97.2 | 90.9 |
| Redundancy | 4.8 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 298 | NH4H2PO4, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 16 (%) | |
| 2 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |
| 3 | 1 | reservoir | 1,4-dioxane | 5 (%) | |
| 4 | 1 | drop | protein | 15 (mg/ml) |
