1PQ0
Crystal structure of mouse Bcl-xl
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-09 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 63.610, 63.610, 109.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.200 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1af3 Rat Bcl-xl |
RMSD bond length | 0.006 |
RMSD bond angle | 1.090 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.340 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.087 | 0.285 * |
Number of reflections | 11386 | 968 * |
<I/σ(I)> | 9.4 | |
Completeness [%] | 95.7 | 88.4 * |
Redundancy | 4.1 * | 3.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 298 | NH4SO4, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 16 (%) | |
2 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |
3 | 1 | reservoir | 1,4-dioxane | 5 (%) | |
4 | 1 | drop | protein | 15 (mg/ml) |