1PQ0

Crystal structure of mouse Bcl-xl

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-09
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	63.610, 63.610, 109.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.200
R-factor	0.217
Rwork	0.217
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1af3 Rat Bcl-xl
RMSD bond length	0.006
RMSD bond angle	1.090 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.340
High resolution limit [Å]	2.200	2.200
Rmerge	0.087	0.285 *
Number of reflections	11386	968 *
<I/σ(I)>		9.4
Completeness [%]	95.7	88.4 *
Redundancy	4.1 *	3.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5 *	298	NH4SO4, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	16 (%)
2	1	reservoir	MES	0.1 (M)	pH6.5
3	1	reservoir	1,4-dioxane	5 (%)
4	1	drop	protein	15 (mg/ml)