1PPR

PERIDININ-CHLOROPHYLL-PROTEIN OF AMPHIDINIUM CARTERAE

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	283
Detector technology	IMAGE PLATE
Collection date	1995-05-16
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	198.426, 116.296, 67.025
Unit cell angles	90.00, 94.89, 90.00

Refinement procedure

Resolution	40.000 - 2.000
R-factor	0.179
Rwork	0.179
R-free	0.20100
Structure solution method	SIR
RMSD bond length	0.012
RMSD bond angle	22.390 *
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.800	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.054	0.265 *
Number of reflections	96045
Completeness [%]	92.5	82.4 *
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.8	17 *	pH 5.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	PEG8000	4-6 (%)
3	1	drop		100 (mM)
4	1	drop		50 (mM)
5	1	drop	triethylammoniumphosphate	24 (mM)
6	1	drop	Tris-HCl	50 (mM)
7	1	reservoir	PEG8000	8-12 (%)