1PPP

CRYSTAL STRUCTURE OF PAPAIN-E64-C COMPLEX. BINDING DIVERSITY OF E64-C TO PAPAIN S2 AND S3 SUBSITES

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	43.370, 102.340, 49.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 1.900
R-factor	0.194
RMSD bond length	0.049
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.087 *
Total number of observations	21135 *
Number of reflections	14811 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	9.2 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	1.5 (%(w/v))
2	1	drop		76 (mM)
3	1	drop	methanol	44 (%(v/v))
4	1	drop	ethanol	22 (%(v/v))
5	1	reservoir	aminoethanol-ethanol	0.1 (M)
6	1	reservoir	methanol	44 (%(v/v))
7	1	reservoir	ethanol	22 (%(v/v))