1PPI

THE ACTIVE CENTER OF A MAMMALIAN ALPHA-AMYLASE. THE STRUCTURE OF THE COMPLEX OF A PANCREATIC ALPHA-AMYLASE WITH A CARBOHYDRATE INHIBITOR REFINED TO 2.2 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	56.300, 87.800, 103.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.153
Rwork	0.153
RMSD bond length	0.015
RMSD bond angle	2.900
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.060 *
Rmerge	0.036 *
Total number of observations	93924 *
Number of reflections	29212 *
Completeness [%]	95.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	1		1 (mM)
3	1	1	amylase
4	1	1	Tris-HCl	10 (mM)