1PPA

THE CRYSTAL STRUCTURE OF A LYSINE 49 PHOSPHOLIPASE A2 FROM THE VENOM OF THE COTTONMOUTH SNAKE AT 2.0 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	71.530, 71.530, 57.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.157 *
Rwork	0.157
Refinement software	CEDAR

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.080 *
Number of reflections	10136 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir	ammonium sulfate	2.0-2.3 (M)
3	1	reservoir	CHES	50 (mM)
4	1	reservoir	dioxane	2 (%(v/v))