1PP8
crystal structure of the T. vaginalis IBP39 Initiator binding domain (IBD) bound to the alpha-SCS Inr element
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.96 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 292.000, 292.000, 292.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 146.000 - 3.050 |
| R-factor | 0.269 |
| Rwork | 0.269 |
| R-free | 0.31100 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 22.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 146.000 | 3.160 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Number of reflections | 36349 | |
| <I/σ(I)> | 7.3 | 1.8 |
| Completeness [%] | 93.0 | 95.1 |
| Redundancy | 4.4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | Ammonium sulphate, MES pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ammonium sulphate | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | Ammonium sulphate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 2.5 (M) | |
| 3 | 1 | reservoir | MES | 0.1 (M) | pH5.6 |
