1PP7
Crystal structure of the T. vaginalis Initiator binding protein bound to the ferredoxin Inr
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.06 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 81.340, 41.430, 58.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.430 - 2.450 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | one of the IBD-alphaSCS complexes that is being deposited simultaneous with this deposition |
RMSD bond length | 0.017 |
RMSD bond angle | 21.800 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.430 | 2.510 |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 7158 | |
<I/σ(I)> | 5.7 | |
Completeness [%] | 94.5 | 98.4 |
Redundancy | 2.6 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6 | 298 | PEG 8000, MES pH 6.0, 0.1 M Zinc acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 8000 | ||
2 | 1 | 1 | MES | ||
3 | 1 | 1 | Zinc acetate | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | PEG 8000 | ||
6 | 1 | 2 | Zinc acetate |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 10 (%) | |
2 | 1 | reservoir | 0.1 (M) | pH6.0 | |
3 | 1 | reservoir | zinc acetate | 0.1 (M) |