1POX

THE REFINED STRUCTURES OF A STABILIZED MUTANT AND OF WILD-TYPE PYRUVATE OXIDASE FROM LACTOBACILLUS PLANTARUM

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	121.600, 155.400, 167.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.162
Rwork	0.162
RMSD bond length	0.014
RMSD bond angle	2.700
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.100 *
Rmerge	0.122 *
Total number of observations	280000 *
Number of reflections	88000 *
Completeness [%]	95.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.2 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	20 (mg/ml)
2	1	drop	potasssium phosphate	50 (mM)
3	1	drop	glycerol	5 (%(v/v))
4	1	drop	ammonium sulphate	0.5 (M)
5	1	drop	ThDP	1 (mM)
6	1	reservoir	ammonium sulfate	0.95 (M)
7	1	reservoir	potassium phosphate	50 (mM)
8	1	reservoir	glycerol	10 (%(v/v))