1POH

THE 2.0 ANGSTROMS RESOLUTION STRUCTURE OF ESCHERICHIA COLI HISTIDINE-CONTAINING PHOSPHOCARRIER PROTEIN HPR: A REDETERMINATION

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	27.470, 45.950, 25.860
Unit cell angles	90.00, 104.39, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.135
Rwork	0.135
RMSD bond length	0.021
RMSD bond angle	0.054 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.000 *
Rmerge	0.030 *
Total number of observations	20532 *
Number of reflections	4023 *
Completeness [%]	94.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	3.7 *	14 *	referred to 'El-Kabbani', (1993) J.Biol.Chem., 262, 12926-12929 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	lithium sulfate	68 (%sat)
2	1	1	citrate	0.1 (M)
3	1	1		0.2 (M)