1PNE
CRYSTALLIZATION AND STRUCTURE DETERMINATION OF BOVINE PROFILIN AT 2.0 ANGSTROMS RESOLUTION
Experimental procedure
| Collection date | 1993 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.150, 34.590, 52.490 |
| Unit cell angles | 90.00, 92.56, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.165 |
| Rwork | 0.165 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.990 * |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.066 |
| Total number of observations | 29297 * |
| Number of reflections | 8239 |
| Completeness [%] | 95.9 |
| Redundancy | 3.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 1.5 (%) | |
| 2 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 3 | 1 | reservoir | beta-mercaptoethanol | 10 (mM) | |
| 4 | 1 | drop | HEPES | 0.1 (M) | |
| 5 | 1 | drop | profilin | 14 (mg/ml) |
