1PN3

Crystal Structure of TDP-epi-Vancosaminyltransferase GtfA in complexes with TDP and the acceptor substrate DVV.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-03
Detector	ADSC QUANTUM 315
Spacegroup name	P 32 2 1
Unit cell lengths	152.500, 152.500, 98.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.212
Rwork	0.212
R-free	0.25000 *
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	22.970 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (V. 2.0)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.081 *	0.295 *
Number of reflections	31308
<I/σ(I)>	15.1
Completeness [%]	99.2 *	99.8
Redundancy	7	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.1		NA,K PHOSPHATE, PH 6.1, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium potassium phosphate	1.3 (M)	pH6.1