1PMB

THE DETERMINATION OF THE CRYSTAL STRUCTURE OF RECOMBINANT PIG MYOGLOBIN BY MOLECULAR REPLACEMENT AND ITS REFINEMENT

Experimental procedure

Spacegroup name	I 1 21 1
Unit cell lengths	124.910, 42.630, 92.200
Unit cell angles	90.00, 92.16, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.185
RMSD bond length	0.024
RMSD bond angle	0.075
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.059 *
Total number of observations	49979 *
Number of reflections	15941 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.1 *	15 *	taken from Dodson, G.(1988). Protein. Eng., 2, 233-237. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	sodium phosphate	50 (mM)
3	1	reservoir	ammonium sulphate	80 (%)