1PM9
CRYSTAL STRUCTURE OF HUMAN MNSOD H30N, Y166F MUTANT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-12-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 71.580, 76.460, 66.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.700 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.24000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ABM |
RMSD bond length | 0.006 |
RMSD bond angle | 20.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.060 | 0.380 * |
Number of reflections | 40814 | |
<I/σ(I)> | 18.6 | 3 |
Completeness [%] | 99.0 | 96 * |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 298 | potassium, PEG 3350, pH 7.6, VAPOR DIFFUSION, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | reservoir | potassium phosphate | 25 (mM) | pH7.6 |
3 | 1 | reservoir | PEG3350 | 25 (%) |