1PM5
Crystal structure of wild type Lactococcus lactis Fpg complexed to a tetrahydrofuran containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-14 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 91.784, 91.784, 141.792 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.950 |
Rwork | 0.196 |
R-free | 0.21620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nnj |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (VERSION 1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.000 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 43717 | |
<I/σ(I)> | 10.2 | 4 |
Completeness [%] | 97.5 | 97.5 |
Redundancy | 6.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | citrate, hepes, glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | citrate | ||
2 | 1 | 1 | hepes | ||
3 | 1 | 1 | glycerol | ||
4 | 1 | 1 | water | ||
5 | 1 | 2 | citrate | ||
6 | 1 | 2 | glycerol |