1PM4
Crystal structure of Yersinia pseudotuberculosis-derived mitogen (YPM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.071 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.651, 78.652, 32.901 |
| Unit cell angles | 90.00, 91.97, 90.00 |
Refinement procedure
| Resolution | 40.000 * - 1.750* |
| R-factor | 0.18438 |
| Rwork | 0.182 |
| R-free | 0.22900 * |
| Structure solution method | MAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 * |
| Rmerge | 0.045 | 0.515 |
| Total number of observations | 253092 * | |
| Number of reflections | 35166 * | |
| <I/σ(I)> | 39.1 | 3.2 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 * | 293 | Donadini, R., (2003) Acta Cryst., D59, 1330. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.95-2.10 (M) | |
| 2 | 1 | reservoir | PEG200 | 4-5 (%(v/v)) | |
| 3 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.8-7.6 |
| 4 | 1 | drop | protein | 75 (mg/ml) | |
| 5 | 1 | drop | sodium citrate | 0.1 (M) | pH7.2 |
