1PLU

PECTATE LYASE C FROM ERWINIA CHRYSANTHEMI WITH 1 LU+3 ION IN THE PUTATIVE CALCIUM BINDING SITE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	291
Detector technology	AREA DETECTOR
Collection date	1992-06
Detector	SDMS
Spacegroup name	P 21 21 21
Unit cell lengths	72.600, 80.600, 95.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.221
Rwork	0.221
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PREVIOUS PELC MODEL
RMSD bond length	0.007
RMSD bond angle	1.190 *
Data reduction software	SDMS (SOFTWARE)
Data scaling software	SDMS
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.200
Number of reflections	24983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.9	4 *	PROTEIN WAS CRYSTALLIZED FROM 1.0MM LUCL3, 1.0M AMMONIUM SULFATE, AND 50MM HEPES, PH 6.9

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (A280/ml)
2	1	drop		1.0 (M)
3	1	drop	HEPES	50 (mM)
4	1	drop		1 (mM)
5	1	reservoir		2.0 (M)
6	1	reservoir	HEPES	0.1 (M)