1PLK

CRYSTALLOGRAPHIC STUDIES ON P21H-RAS USING SYNCHROTRON LAUE METHOD: IMPROVEMENT OF CRYSTAL QUALITY AND MONITORING OF THE GTPASE REACTION AT DIFFERENT TIME POINTS

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	40.300, 40.300, 158.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.800
R-factor	0.2277
Rwork	0.228
RMSD bond length	0.030
RMSD bond angle	4.421
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.087 *
Total number of observations	8723 *
Number of reflections	2590 *
Completeness [%]	65.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)
2	1	drop	n-octyl-beta-D-glucopyranoside	0.01-0.05 (%(w/v))
3	1	drop	DTE	50 (mM)
4	1	drop	PEG400	27-29 (%(w/v))
5	1	reservoir	PEG400	30-34 (%(w/v))