1PLC

ACCURACY AND PRECISION IN PROTEIN CRYSTAL STRUCTURE ANALYSIS: RESTRAINED LEAST-SQUARES REFINEMENT OF THE CRYSTAL STRUCTURE OF POPLAR PLASTOCYANIN AT 1.33 ANGSTROMS RESOLUTION

Replaces:  1PCY

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	29.600, 46.860, 57.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.330
R-factor	0.15
RMSD bond length	0.016
RMSD bond angle	0.032
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.330 *
Rmerge	0.100 *
Total number of observations	14512 *
Number of reflections	8460 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6 *		taken from Chapman, G.V. et al (1977). J. Mol. Biol., 110, 187-189. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-10 (mg/ml)
2	1	reservoir	sodium phosphate	0.1 (M)
3	1	reservoir	ammonium sulfate	2.6 (M)