1PL9
Crystal structure of KDO8P synthase in its binary complex with substrate analog Z-FPEP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-10 |
| Detector | MACSCIENCE |
| Wavelength(s) | 0.934 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 117.055, 117.055, 117.055 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.28 |
| Rwork | 0.233 |
| R-free | 0.32710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1D9E (a) |
| RMSD bond length | 1.718 |
| RMSD bond angle | 0.009 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.890 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.102 | 0.110 |
| Number of reflections | 6309 | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 96.2 | 96.7 |
| Redundancy | 4.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 294 | EG 4000, Glycrol, Tris-HCl , pH 7.4 , VAPOR DIFFUSION, HANGING DROP, temperature 294 K |
