1PL8
human SDH/NAD+ complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-18 |
| Detector | BRANDEIS - B1.2 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 62 |
| Unit cell lengths | 133.776, 133.776, 224.708 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.800 * - 1.900 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 23.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.083 * | 0.379 |
| Number of reflections | 161025 | |
| <I/σ(I)> | 12.8 | 2 |
| Completeness [%] | 88.6 | 44.2 |
| Redundancy | 4 * | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.15 | 295 | NH4AC, NaCitrate, PEG 4000, pH 6.15, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG4000 | 28 (%) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | NAD+ | 0.2 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 26.5 (%) | |
| 5 | 1 | reservoir | 10 (mM) |
