1PL5

Crystal Structure Analysis of the Sir4p C-terminal Coiled Coil

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	93
Wavelength(s)	1.0
Spacegroup name	P 65
Unit cell lengths	108.884, 108.884, 75.342
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.150 - 2.500
R-factor	0.261
Rwork	0.261
R-free	0.28800
Structure solution method	SIR
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.150	2.170
High resolution limit [Å]	2.100	2.100
Number of reflections	28687
<I/σ(I)>	15.4	0.16
Completeness [%]	96.7	94.6
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	calcium chloride, sodium chloride, Tris, ethylene glycol, sodium azide, dithiothreitol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K