1PKQ
Myelin Oligodendrocyte Glycoprotein-(8-18C5) Fab-complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0500 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 124.480, 40.000, 134.200 |
| Unit cell angles | 90.00, 107.75, 90.00 |
Refinement procedure
| Resolution | 18.000 - 3.000 |
| Rwork | 0.250 |
| R-free | 0.31900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.660 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE (& BEAST) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 3.090 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.094 | 0.351 |
| Number of reflections | 22490 * | |
| <I/σ(I)> | 5.3 | 2 |
| Completeness [%] | 86.7 | 73 |
| Redundancy | 2.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 18 * | Tris/HCl, MOPS, NaCl, PEG8000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.5 (mg/ml) | |
| 2 | 1 | drop | MOPS | 5 (mM) | pH7.5 |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5 |
| 5 | 1 | reservoir | PEG8000 | 14 (%) |
