1PKO

Myelin Oligodendrocyte Glycoprotein (MOG)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-09
Detector	MARRESEARCH
Wavelength(s)	1.0500
Spacegroup name	P 32 2 1
Unit cell lengths	50.234, 50.234, 76.610
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.450
Rwork	0.194
R-free	0.21900
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.640
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.470
High resolution limit [Å]	1.450	1.450
Rmerge	0.051	0.398
Number of reflections	20292 *
<I/σ(I)>	28.1	3.9
Completeness [%]	99.3	99.6
Redundancy	3.9	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	18 *	Tris/HCl, PEG8000, MgCl2, NaCl, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 291K
1	VAPOR DIFFUSION, SITTING DROP	7.4	18 *	Tris/HCl, PEG8000, MgCl2, NaCl, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)	pH7.4
3	1	drop		30 (mM)
4	1	reservoir	Tris-HCl	100 (mM)	pH7.2
5	1	reservoir		100 (mM)
6	1	reservoir	PEG8000	22 (%)