1PKM
THE REFINED THREE-DIMENSIONAL STRUCTURE OF CAT MUSCLE (M1) PYRUVATE KINASE, AT A RESOLUTION OF 2.6 ANGSTROMS
Experimental procedure
| Detector technology | FILM |
| Collection date | 1978-01-01 |
| Detector | FILM |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 88.400, 115.300, 131.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 * - 2.600 |
| R-factor | 0.175 * |
| Rwork | 0.175 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.690 |
| Data reduction software | MOSCO |
| Phasing software | X-PLOR |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Rmerge | 0.050 |
| Number of reflections | 15440 * |
| Completeness [%] | 74.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 * | Stammers, D.K., (1975) J. Mol. Biol., 95, 213. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 13-17 (mg/ml) | |
| 2 | 1 | 2 | ammonium sulfate | 50 (%sat) | |
| 3 | 1 | 2 | sodium phosphate | 20 (mM) |
