1PKK
Structural basis for recognition and catalysis by the bifunctional dCTP deaminase and dUTPase from Methanococcus jannaschii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-18 |
Detector | ADSC QUANTUM |
Wavelength(s) | 0.980 |
Spacegroup name | P 21 3 |
Unit cell lengths | 110.576, 110.576, 110.576 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.100 - 1.770 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.21900 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.830 |
High resolution limit [Å] | 1.770 | 1.770 |
Number of reflections | 44147 | |
<I/σ(I)> | 31.1 | 4.1 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | nanopure water, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |