1PKG

Structure of a c-Kit Kinase Product Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	92.667, 116.458, 60.066
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.900
R-factor	0.22874
Rwork	0.225
R-free	0.31000 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.610
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.900	2.900 *
Rmerge	0.072 *	0.424 *
Total number of observations	49361 *
Number of reflections	14652
Completeness [%]	97.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.9 *	295	PEG, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	6 (mg/ml)
2	1	drop		150 (mM)
3	1	drop	Tris	25 (mM)	pH7.9
4	1	drop	ATP	2.5 (mM)
5	1	drop		5 (mM)
6	1	reservoir	PEG8000	18 (%)
7	1	reservoir	MES	0.1 (M)	pH7.1