1PK5
Crystal structure of the orphan nuclear receptor LRH-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-26 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.775, 127.542, 53.213 |
Unit cell angles | 90.00, 91.72, 90.00 |
Refinement procedure
Resolution | 24.550 - 2.400 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.23100 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | RXR ALPHA (PDB ID 1FBY) |
RMSD bond length | 0.009 * |
RMSD bond angle | 1.570 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.550 |
High resolution limit [Å] | 2.400 * | 2.400 |
Rmerge | 0.067 | 0.169 |
Number of reflections | 17954 * | |
<I/σ(I)> | 24.4 | 5.7 |
Completeness [%] | 98.2 * | 88.8 * |
Redundancy | 6 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8.8 | 15 * | PEG 4000, glycerol, TRIS, isopropanol, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | reservoir | glycerol | 15 (%) | |
3 | 1 | reservoir | PEG4000 | 21 (%) | |
4 | 1 | reservoir | Tris | 100 (mM) | pH8.8 |
5 | 1 | reservoir | isopropanol | 5 (%) |