1PK5

Crystal structure of the orphan nuclear receptor LRH-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-26
Detector	ADSC QUANTUM 210
Wavelength(s)	1.1
Spacegroup name	P 1 21 1
Unit cell lengths	34.775, 127.542, 53.213
Unit cell angles	90.00, 91.72, 90.00

Refinement procedure

Resolution	24.550 - 2.400
R-factor	0.208
Rwork	0.208
R-free	0.23100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	RXR ALPHA (PDB ID 1FBY)
RMSD bond length	0.009 *
RMSD bond angle	1.570 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.550
High resolution limit [Å]	2.400 *	2.400
Rmerge	0.067	0.169
Number of reflections	17954 *
<I/σ(I)>	24.4	5.7
Completeness [%]	98.2 *	88.8 *
Redundancy	6	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.8	15 *	PEG 4000, glycerol, TRIS, isopropanol, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 288K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	glycerol	15 (%)
3	1	reservoir	PEG4000	21 (%)
4	1	reservoir	Tris	100 (mM)	pH8.8
5	1	reservoir	isopropanol	5 (%)