1PK1
Hetero SAM domain structure of Ph and Scm.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 128 |
| Detector technology | CCD |
| Collection date | 2002-02-11 |
| Wavelength(s) | 0.9795, 0.9797, 0.9720, 1.70 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.277, 46.866, 123.987 |
| Unit cell angles | 90.00, 90.72, 90.00 |
Refinement procedure
| Resolution | 19.560 - 1.800 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.24900 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 28975 | |
| Completeness [%] | 89.6 | 76.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 1000, ammonium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 1000, ammonium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
