1PJQ
Structure and function of CysG, the multifunctional methyltransferase/dehydrogenase/ferrochelatase for siroheme synthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.734, 121.493, 130.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.210 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.27500 * |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.950 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.260 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.057 * | 0.453 * |
| Number of reflections | 48472 * | |
| <I/σ(I)> | 23 | 3 |
| Completeness [%] | 99.5 | 97.5 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.5 * | 293 | PEG200, acetate, sodium chloride, 2-mercaptoethanol, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | reservoir | PEG200 | 30 (%(v/v)) | |
| 3 | 1 | reservoir | sodium acetate | 200 (mM) | pH4.5 |
| 4 | 1 | reservoir | 200 (mM) | ||
| 5 | 1 | reservoir | beta-mercaptoethanol | 1 (%(v/v)) |
