1PJI
Crystal structure of wild type Lactococcus lactis FPG complexed to a 1,3 propanediol containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-05-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 91.955, 91.955, 142.076 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.900 |
Rwork | 0.210 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kfv |
RMSD bond length | 0.005 |
RMSD bond angle | 1.240 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (version 1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 48727 | |
<I/σ(I)> | 16.5 | 1 |
Completeness [%] | 99.2 | 99 |
Redundancy | 2.5 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | citrate, Hepes, glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | citrate | ||
2 | 1 | 1 | Hepes | ||
3 | 1 | 1 | glycerol | ||
4 | 1 | 1 | water | ||
5 | 1 | 2 | citrate | ||
6 | 1 | 2 | glycerol |