1PJ7
Structure of dimethylglycine oxidase of Arthrobacter globiformis in complex with folinic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-08-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 70.621, 223.740, 119.371 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.16253 |
Rwork | 0.159 |
R-free | 0.22200 * |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1pj6 |
RMSD bond length | 0.021 |
RMSD bond angle | 1.837 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.08) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.073 | 0.344 * |
Number of reflections | 46564 | |
<I/σ(I)> | 6.2 | |
Completeness [%] | 88.3 | 84.5 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 15% PEG 2000MME, 0.2M MgCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | PEG5000 MME | 15 (%) | |
3 | 1 | reservoir | 0.2 (M) | pH7.5 | |
4 | 1 | reservoir | HEPES | 0.1 (M) |