1PIW
APO AND HOLO STRUCTURES OF AN NADP(H)-DEPENDENT CINNAMYL ALCOHOL DEHYDROGENASE FROM SACCHAROMYCES CEREVISIAE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.939 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 234.980, 100.350, 52.490 |
| Unit cell angles | 90.00, 92.18, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.253 |
| Rwork | 0.218 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 24236 |
| Completeness [%] | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | PEG 4000, MPD, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
