1PIG
PIG PANCREATIC ALPHA-AMYLASE COMPLEXED WITH THE OLIGOSACCHARIDE V-1532
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 275 |
Detector technology | IMAGE PLATE |
Collection date | 1995-07-20 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.500, 114.800, 118.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.000 - 2.200 |
R-factor | 0.178 |
Rwork | 0.178 |
R-free | 0.21000 |
Structure solution method | DIFFERENCE FOURIER |
Starting model (for MR) | 1pif |
RMSD bond length | 0.007 |
RMSD bond angle | 25.000 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.500 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.123 | |
Number of reflections | 47260 | |
Completeness [%] | 93.7 | 97.8 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | VAPOR DIFFUSION; 10 MICROLITER OF PROTEIN (15 MG/ML IN 0.1 M AMMONIUM CACODYLATE, PH 10.0 0.001 M V-1532) WERE STEPWISE CONCENTRATED OVER 0.1, 0.15, AND 0.25 M AMMONIUM CACODYLATE, PH 6.0., vapor diffusion |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25 (mg/ml) | |
2 | 1 | drop | cacodylate | 0.01 (M) | |
3 | 1 | drop | 0.002 (M) |