1PIE

Crystal Structure of Lactococcus lactis Galactokinase Complexed with Galactose

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-04-04
Detector	SBC-2
Wavelength(s)	0.97932, 0.97951, 0.96411, 0.65255
Spacegroup name	I 2 3
Unit cell lengths	143.400, 143.400, 143.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.204
Rwork	0.199
R-free	0.26600
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	18.100 *
Data reduction software	HKL-2000
Data scaling software	d*TREK
Phasing software	SOLVE
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.067 *	0.214 *
Number of reflections	28616	2804 *
<I/σ(I)>	75.4	9.3
Completeness [%]	98.7	99.5
Redundancy	14	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.5 *	277	sodium phosphate, potassium phosphate, CHES, galactose, pH 9, batch, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	HEPES	10 (mM)	pH7.5
2	1	1		200 (mM)
3	1	2	protein	15 (mg/ml)