1PI2

REACTIVE SITES OF AN ANTICARCINOGENIC BOWMAN-BIRK PROTEINASE INHIBITOR ARE SIMILAR TO OTHER TRYPSIN INHIBITORS

Experimental procedure

Spacegroup name	P 41 3 2
Unit cell lengths	89.960, 89.960, 89.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 2.500
R-factor	0.236
RMSD bond length	0.021 *
RMSD bond angle	2.800
Refinement software	GPRLSA

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.300 *
Rmerge	0.078 *
Total number of observations	75913 *
Completeness [%]	95.1 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	soium acetate-acetic acid	0.5 (M)
2	1	drop	protein	0.03 (mg/ml)
3	1	drop	sodium chloride	0.2 (%)
4	1	drop	PEG4000	1.8 (%(w/v))
5	1	drop	sodium azide	0.09 (%)
6	1	drop	ammonium sulfate
7	1	reservoir	ammonium sulfate	40 (%sat)