1PHQ
Crystal structure of KDO8P synthase in its binary complex with substrate analog E-FPEP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.81 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 117.783, 117.783, 117.783 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.2506 |
| Rwork | 0.235 |
| R-free | 0.32170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1D9E (a) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.408 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.036 | 0.246 |
| Number of reflections | 6714 | |
| <I/σ(I)> | 13.9 | 91 |
| Completeness [%] | 88.2 | 91 |
| Redundancy | 6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | PEG 4000, Glycrol, Tris-HCl , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293 K |
