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STRUCTURE OF THE ADP COMPLEX OF THE 3-PHOSPHOGLYCERATE KINASE FROM BACILLUS STEAROTHERMOPHILUS AT 1.65 ANGSTROMS

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	40.410, 73.930, 68.570
Unit cell angles	90.00, 99.80, 90.00

Refinement procedure

Resolution	10.000 - 1.650
R-factor	0.156
Rwork	0.156
RMSD bond length	0.019
RMSD bond angle	0.049 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	1.650 *	1.650 *
Rmerge	0.056 *	0.156 *
Total number of observations	119169 *
Number of reflections	44754 *
Completeness [%]	94.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	Tris-HCl	8 (mM)
3	1	drop	Mg-ATP	1 (mM)
4	1	drop		0.1 (mM)
5	1	drop	PEG600	10 (%(w/v))
6	1	reservoir	PEG600	40 (%(w/v))