1PHH

CRYSTAL STRUCTURE OF P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH ITS REACTION PRODUCT 3,4-DIHYDROXYBENZOATE

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	71.900, 146.300, 88.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 2.300
R-factor	0.193
RMSD bond length	0.013
RMSD bond angle	2.100 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Total number of observations	103934 *
Number of reflections	15546 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.5 *	4-20 *	took method of Drenth et al., 1975 from original paper *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	5 (mg/ml)
2	1	1	p-hydroxybezoate	2 (mM)
3	1	1	FAD	0.04 (mM)
4	1	1	EDTA	0.3 (mM)
5	1	1	reduced glutathione	0.2 (mM)
6	1	1	sodium sulfate	30 (mM)
7	1	1	potassium phosphate	0.1 (M)