1PHC
CRYSTAL STRUCTURE OF SUBSTRATE-FREE PSEUDOMONAS PUTIDA CYTOCHROME P450
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 108.670, 103.900, 36.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 1.600 |
| R-factor | 0.193 * |
| RMSD bond length | 0.022 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Rmerge | 0.068 * |
| Total number of observations | 122589 * |
| Number of reflections | 18439 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 * | 12-15 * | P-450CAM was prepared in solutions containing 1 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 50 (mM) | ||
| 2 | 1 | 1 | 250 (mM) | ||
| 3 | 1 | 1 | dithiothreitol | 50 (mM) | |
| 4 | 1 | 2 | ammonium sulphate | 66-70 (%sat) |
