1PH9
CRYSTAL STRUCTURE OF THE OXYTRICHA NOVA TELOMERE END-BINDING PROTEIN COMPLEXED WITH NONCOGNATE SSDNA GGGGTTTTGAGG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 83 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 92.972, 92.972, 422.119 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.750 - 2.500 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 24.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.750 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.096 * |
Number of reflections | 29531 |
Completeness [%] | 76.2 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6 * | 277 | drop contains the same amount of reservoir solution except 50mM NaCl * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | alpha subunit | 0.2 (M) | |
10 | 1 | reservoir | PEG4000 | 3-8 (%) | |
11 | 1 | reservoir | MES | 40 (mM) | |
12 | 1 | reservoir | 0.02 (%) | ||
13 | 1 | reservoir | dithiothreitol | 2 (mM) | |
14 | 1 | reservoir | 50 (mM) | ||
2 | 1 | drop | beta subunit | 1.0-1.5 equivalents each of | |
3 | 1 | drop | ssDNA | ||
4 | 1 | drop | 50 (mM) | ||
5 | 1 | drop | Tris-HCl | 5 (mM) | |
6 | 1 | drop | EDTA | 0.1 (mM) | |
7 | 1 | drop | 0.02 (%) | ||
8 | 1 | drop | dithiothreitol | 2 (mM) | |
9 | 1 | reservoir | ethylene glycol | 30 (%) |