1PGX
THE 1.66 ANGSTROMS X-RAY STRUCTURE OF THE B2 IMMUNOGLOBULIN-BINDING DOMAIN OF STREPTOCOCCAL PROTEIN G AND COMPARISON TO THE NMR STRUCTURE OF THE B1 DOMAIN
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.040, 34.500, 35.950 |
| Unit cell angles | 90.00, 100.84, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.660 |
| R-factor | 0.191 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 0.028 |
| Refinement software | PROFFT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.660 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium citrate | 30 (mM) | |
| 2 | 1 | drop | CaCl2 | 10 (mM) | |
| 3 | 1 | drop | PEG-3350 | 10 (%) | |
| 4 | 1 | drop | octyl beta-glucoside | 10 (mM) | |
| 5 | 1 | drop | protein | 17.5 (mg/ml) | |
| 6 | 1 | reservoir | PEG-3350 | 25 (%) |
