1PGS

THE THREE-DIMENSIONAL STRUCTURE OF PNGASE F, A GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	85.070, 85.140, 48.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.168
RMSD bond length	0.015 *
RMSD bond angle	1.900 *
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	0.050 *
Total number of observations	143717 *
Number of reflections	28025 *
Completeness [%]	84.4 *	65 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	0.1 (M)
3	1	drop	PEG4000	12 (%)
4	1	reservoir	Tris-HCl	0.1 (M)
5	1	reservoir	PEG4000	18 (%(w/v))