1PGO

CRYSTALLOGRAPHIC STUDY OF COENZYME, COENZYME ANALOGUE AND SUBSTRATE BINDING IN 6-PHOSPHOGLUCONATE DEHYDROGENASE: IMPLICATIONS FOR NADP SPECIFICITY AND THE ENZYME MECHANISM

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	72.740, 148.400, 102.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.500
R-factor	0.17
Rwork	0.170
RMSD bond length	0.011
RMSD bond angle	2.530
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.067 *
Total number of observations	46279 *
Number of reflections	17995 *
Completeness [%]	92.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *			Somers, D.O., (1991) Protein Expr. Purif., 2, 385. *