1PGJ

X-RAY STRUCTURE OF 6-PHOSPHOGLUCONATE DEHYDROGENASE FROM THE PROTOZOAN PARASITE T. BRUCEI

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1992-11
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	135.130, 135.130, 116.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.760 - 2.820
R-factor	0.186
Rwork	0.186
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SHEEP DIMER - SEE PDB ENTRY 2PGD
RMSD bond length	0.013
RMSD bond angle	1.700
Data reduction software	MOSFLM
Data scaling software	SSM
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800
Rmerge	0.099 *
Total number of observations	212041 *
Number of reflections	29373
<I/σ(I)>	17.9
Completeness [%]	95.9
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7		Barrett, M.P., (1994) Protein Expr. Purif., 5, 44. *