1PGB
TWO CRYSTAL STRUCTURES OF THE B1 IMMUNOGLOBULIN-BINDING DOMAIN OF STREPTOCCOCAL PROTEIN G AND COMPARISON WITH NMR
Experimental procedure
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 36.633, 36.633, 79.254 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 1.920 |
| R-factor | 0.198 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.045 |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.860 * |
| Rmerge | 0.068 * |
| Number of reflections | 5014 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4 * | 18 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 70 (%) | |
| 2 | 1 | reservoir | 300 (mM) | ||
| 3 | 1 | reservoir | NaOAc | 25 (mM) | |
| 4 | 1 | drop | protein | 10 (mg/ml) |
