1PG7

Murine 6A6 Fab in complex with humanized anti-Tissue Factor D3H44 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2001-12-21
Detector	CUSTOM-MADE
Wavelength(s)	0.917
Spacegroup name	P 1
Unit cell lengths	78.890, 85.780, 92.370
Unit cell angles	77.48, 75.92, 63.33

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.217
Rwork	0.217
R-free	0.26700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1JPS 1gig
RMSD bond length	0.009
RMSD bond angle	28.100 *
Data reduction software	HKL-2000
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	AMoRE
Refinement software	X-PLOR (98.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.129	0.477
Total number of observations	310288 *
Number of reflections	72248	7157 *
<I/σ(I)>	12
Completeness [%]	99.0	98
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	4 *	PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	18 (mg/ml)
2	1	drop	Tris-HCl	25 (mM)	pH8.0
3	1	reservoir	PEG3350	20 (%)
4	1	reservoir	Tris	100 (mM)	pH7.0