1PFR
RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE 1 BETA CHAIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1992-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.300, 85.500, 115.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.200 |
| R-factor | 0.179 * |
| Rwork | 0.179 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DIFERRIC FORM OF R2 PROTEIN PDB ENTRY 1RIB |
| RMSD bond length | 0.011 |
| RMSD bond angle | 17.252 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.095 | 0.376 |
| Total number of observations | 95516 * | |
| Number of reflections | 34220 * | |
| <I/σ(I)> | 7.1 | 1.8 |
| Completeness [%] | 86.0 | 34.1 |
| Redundancy | 2.79 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | HANGING DROP VAPOR DIFFUSION. CRYSTALLISED FROM 20% PEG 4000, 0.2M NACL, 0.1M MES PH 6.0, 1MM ETHYLMERCURY SALICYLATE., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 22 (%) | |
| 2 | 1 | reservoir | 0.2 (M) | ||
| 3 | 1 | reservoir | MES | 50 (mM) | |
| 4 | 1 | reservoir | ethylmercury thiosalicylate | 1 (mM) |
