1PFP
CATHELIN-LIKE MOTIF OF PROTEGRIN-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97992 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 51.541, 51.541, 134.512 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.000 * - 2.300 |
| R-factor | 0.22713 |
| Rwork | 0.219 |
| R-free | 0.28300 * |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.560 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 * | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.078 | 0.295 |
| Total number of observations | 48282 * | |
| Number of reflections | 5162 | |
| <I/σ(I)> | 13.1 | 2.9 |
| Completeness [%] | 99.7 | 98.6 * |
| Redundancy | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.8 | 291 | ammonium sulfate, sodium acetate, pH 3.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 2.1 (M) | |
| 3 | 1 | reservoir | sodium acetate | pH3.8 |
