1PFB
Structural Basis for specific binding of polycomb chromodomain to histone H3 methylated at K27
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 32.429, 77.033, 77.273 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.400 |
| Rwork | 0.197 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.290 * |
| Data reduction software | MADNESS |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.042 * | 0.195 * |
| Total number of observations | 85538 * | |
| Number of reflections | 17050 | 811 * |
| Completeness [%] | 86.8 | 42.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 16 * | 30% PEG8000, 200mM ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris | 5 (%) | pH8.0 |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | dithiothreitol | 1 (mM) | |
| 6 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.5 |
| 7 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 8 | 1 | reservoir | PEG8000 | 30 (%) | |
| 9 | 1 | reservoir | dithiothreitol | 10 (mM) |
